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human hepatoma cell line huh7.5 cells  (Apath LLC)

 
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    Apath LLC human hepatoma cell line huh7.5 cells
    Human Hepatoma Cell Line Huh7.5 Cells, supplied by Apath LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human hepatoma cell line huh7.5 cells/product/Apath LLC
    Average 90 stars, based on 1 article reviews
    human hepatoma cell line huh7.5 cells - by Bioz Stars, 2026-06
    90/100 stars

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    hcv- negative human hepatoma cell line huh7.5 cells  (Apath LLC)
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    ( A <t>)</t> <t>Huh7.5</t> or Huh7.5-FL cells grown for 48 h were stained with antibodies against <t>HCV</t> proteins (HCV core or NS4A), followed by FITC-coupled secondary antibodies and detected using confocal microscopy. ( B ) Lysates of cells grown for 48 hours were analyzed for expression of HCV core, NS4A or NS5B by Western blotting. HepG2 cells were transfected with JFH-1 RNA and expression of HCV NS5B and core protein was analyzed by Western blotting ( C ) and confocal microscopy respectively ( D ). Equal protein loading in the Western blot analyses was verified using GAPDH antibody. Data are from one of three independent experiments performed in triplicate.
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    ( A ) Huh7.5 or Huh7.5-FL cells grown for 48 h were stained with antibodies against HCV proteins (HCV core or NS4A), followed by FITC-coupled secondary antibodies and detected using confocal microscopy. ( B ) Lysates of cells grown for 48 hours were analyzed for expression of HCV core, NS4A or NS5B by Western blotting. HepG2 cells were transfected with JFH-1 RNA and expression of HCV NS5B and core protein was analyzed by Western blotting ( C ) and confocal microscopy respectively ( D ). Equal protein loading in the Western blot analyses was verified using GAPDH antibody. Data are from one of three independent experiments performed in triplicate.

    Journal: PLoS ONE

    Article Title: Activation of the Connective Tissue Growth Factor (CTGF)-Transforming Growth Factor β 1 (TGF-β 1) Axis in Hepatitis C Virus-Expressing Hepatocytes

    doi: 10.1371/journal.pone.0046526

    Figure Lengend Snippet: ( A ) Huh7.5 or Huh7.5-FL cells grown for 48 h were stained with antibodies against HCV proteins (HCV core or NS4A), followed by FITC-coupled secondary antibodies and detected using confocal microscopy. ( B ) Lysates of cells grown for 48 hours were analyzed for expression of HCV core, NS4A or NS5B by Western blotting. HepG2 cells were transfected with JFH-1 RNA and expression of HCV NS5B and core protein was analyzed by Western blotting ( C ) and confocal microscopy respectively ( D ). Equal protein loading in the Western blot analyses was verified using GAPDH antibody. Data are from one of three independent experiments performed in triplicate.

    Article Snippet: In this study we used HCV- negative human hepatoma cell line Huh7.5 cells and Huh7.5 cells harboring full genome length HCV [Con1/FL-Neo HCV1b FL (S2204I)] (Huh7.5-FL) replicon cells (Apath, LLC; St. Louis, MO) and propagated in complete Dulbecco's modified Eagle's medium (DMEM) (Invitrogen, Carlsbad, CA).

    Techniques: Staining, Confocal Microscopy, Expressing, Western Blot, Transfection